Read this entire manual before using the odyssey fc imager. Quantitative cell signaling analysis with odyssey clx. This plug will only fit into a groundingtype outlet. Odyssey clx imaging system minimize variation and maximize precision with highsensitivity nir fluorescent detection optics engineered to enable definitive analysis of protein. Start the odyssey software and select the set calibration data option from the site menu on the next screen select import files. The detection quality is excellent and the software is both easy to use and provides clear quantification. The odyssey user guide and tutorial manual describe the use of this software. Leveraging this platforms capabilities, bioagilytix is able to perform accurate, reproducible, quantitative western blot assays. Although the lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that licor also sells. Please note there is a blemish in the reat of the instrument, pictured, it is cosmetic and wont all of our lab equipment comes backed with our standard warranty. Licor odyssey clx the odyssey clx combines precision laser illumination, large scan area, and high image resolution to deliver accurate, replicable data capture. This video tutorial demonstrates the acquire ribbon, which contains all of the controls for acquiring new images with image studio 4. The licor odyssey clx imager should not be used with any radioactive materials.
From odyssey software odyssey software operation is discussed in this chapter. Licore odyssey clx the azrieli faculty of medicine. These are products by a single manufacturer or a list of manufacturers. Odyssey infrared imaging system tutorial manual v 3.
Manuals, software information, and webinars are also listed. Used licor odyssey sa infrared imaging system 3014309. A multiuser site license for imagequant tl iqtl is available for the analysi sof typhoon data. The licor odyssey western blot delivers quantitative accuracy for target protein detection by using the platforms infrared technology to match or exceed chemiluminescent detection sensitivity. In automatic mode the odyssey clx imager acquires images with virtually no saturated pixels on the first attempt with no user adjustments. Both the recorder and sensor are included in one encapsulated water proof housing for use in air or under water. Since the odyssey is a two channel instrument it can record data for either wavelength separately or both wavelengths simultaneously. Licor biosciences, odyssey infrared imaging system b446. This licor odyssey 9120 infrared imaging system has thoroughly inspected and is in great condition. Licor, odyssey, mpx, incell western, and irdye trademarks contained in the software product are trademarks or registered trademarks of licor, inc. The li180 features multiple software options for flexibility and performance.
Licor odyssey sa infrared imaging system gmi trusted. This allows access to the odyssey imager via the odyssey application software. Licor odyssey clx imaging system the lab world group. The only negative is that the handling of the software could be based more on windowshandling e. The odyssey infrared imaging system allows multiplex western blotting and accurate protein detection and quantification using secondary antibodies labeled with irdye nearinfrared nir fluorescent dyes. The licor odyssey clx imaging system gives your lab clear sharp digital results in as little as 6 minutes. More details about the software image studio lite 5. Odyssey infrared imaging system operators manual v 3. Computer software cables cords manual chemiluminescence and. After physically connecting the odyssey xp to the computer, you have to add the odyssey xp to the list of available plotters in the software. Licor odyssey 9120 gel imaging system used imagers. By using nir fluorescence detection, this imaging system greatly simplifies your western blot process by eliminating the need to incubate your substrate or wait for film. The licor odyssey 9120 infrared imaging system passed all tests and was found to be fully functional. It has two lasers, which operate at 680 and 780nm, for the detection of infrared fluorescent dyes.
We also use it for emsa, cell migration, coomassie stained gel pictures etc. Infrared detection gives you the quantitative analysis and wide linear dynamic range that chemiluminescence and visible fluorescence cannot. Licor lab instruments, equipment, and accessories biosurplus. For more information on the odyssey clx and other imaging systems, reagents and. Quantitative analysis of the spots is fast and easy. Navigate to your cd drive and select the calibration files for your loggers. For conformity information, contact licor support at biosupport outside of the u. Licor biosciences is an international biotech company which designs, manufactures, and markets instruments, measurement systems, and software for biological and environmental research, and develops relevant measurement methodologies and techniques. Used other licor odyssey imager for sale dotmed listing.
Configure continuous or timed capture and visualize your spectrometer data from within the desktop software. Locationofinterlockson theodysseyclxinstrumentbezel. Odyssey classic operators manual licor biosciences. In addition, software functions related to the odyssey. Data from both scanners can also be quantitated with public domain imagej software. Projects, scans, and analyses starting scans chapter 2. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created date. Licor odyssey platform for quantitative western blots. For conformity information, contact licor support at biosupport.
The addition makes the plotter available for cutting. When initial screen has loaded, click on file and select new from the dropdown menu. If you are an omega or ga user, follow the instructions in the reference manual to add the odyssey xp as the default plotter. Odyssey infrared imaging system operators manual v 2. The odyssey clx imager has two modes for dynamic range, automatic and manual. In the prompt box that opens, select browse from the right side. Odyssey clx infrared imaging system from licor biosciences. Odyssey infrared imaging system operators manual version 3. Odyssey clx manuals, application notes, software, and webinars.
Eddy covariance systems for measuring ghg emissions with infrared co2h2o gas analyzers and eddypro flux processing software. Whatever your imaging application the sapphire biomolecular imager and the powerful sapphire capture software will deliver high quality quantifiable data. With odyssey clx infrared imaging system, you can get clearer answers with accurate reproducible western blots and more. Odyssey clx application and technical notes show the versatility of the clx. Quantitative gel and blot imaging and analysis shared. Licor odyssey western blots hi everyone, i have western blot pictures, either as a two channel picture or two separate pictures of the two channels 700800nm. Tested connection to software through tplink switch. The odyssey imager and network switch are designed to operate.
One button scanning from the odyssey front panel is described in chapter 7 and the use of an internet browser for scanning is discussed in the odyssey operators manual. Used for visualizing images of genes, gene fragments, and gene products. Information on odyssey software can be found both in this manual and in the odysseyuser guide. The odyssey fc imager is intended to be used by qualified personnel. However, the sapphire biomolecular imager is a next generation laser scanning system which offers the best of both worlds. Odyssey infrared imaging system operators manual version. About this manual symbols used in this manual 22 manual conventions 22 chapter 3. Can be used to distinguish between normal and cancer cells. Scan origin and size of sample are chosen in software. Solid state diode lasers simultaneously provide light excitation at 685 and 785 nm. The odyssey clx can be used to image other companies products that are employing infrared dye technologies.
Press open and the files will be loaded into the software ready for use. Licor, odyssey, mpx, and irdye trademarks contained in the software. Used licor odyssey 9120 digital imaging system for sale. This product model number 2800 is a cemarked product. The sapphire biomolecular imager quantitative western blots. Licor ir based system is obviously elegant system and more quantitative.
Odyssey software for the analysis of data from the odyssey scanner is available upon demand. Connecting the odyssey imager to a network in the basic con. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Odysseyclximager safetyinterlocks interlocks figure14. What is the best detection system for western blots odyssey clx but cant fit it in the budget. I want to analyze them, doing a density intensity plot over a sample lane. What is the best detection system for western blots 5. Electrical considerations power cords the odyssey clx imager is equipped with a 3wire groundingtype plug. Nearinfrared nir fluorescence detection allows you to quantitate proteins over a much wider linear dynamic range than chemiluminescence can. Available for ios and android mobile operating systems. Description of detection optics and signal processing. Chemiluminescence and fluorescence detection methods are both commonly used in western blot analyses. Licor biosciences pioneering family of irdye infrared dyes are synthesized with reactive functional groups that enable easy covalent coupling to antibodies and other biomolecules. Odyssey clx infrared imaging system operators manual.
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